Studies on the Semisynthetic liquid medium for plague bacillus

Studies on the modification of Dorset - Henly medium for the producrtion of bovine tuberculin

Studies on Neisseria gonorrhoeae cultured in liquid medium.

Experiments were carried out to determine the minimum amount of inoculum of gonococci that is needed to produce growth in ANM liquid medium. This was found to be less than had previously been thought. Minimum inocula were used to study penicillin sensitivity in liquid medium. This work showed that growth occurred at higher concentrations of penicillin with larger inocula. Evidence was also obta...

Semisynthetic Penicillin: In Vitro Studies

CI-867, a new semisynthetic penicillin, has exhibited broad-spectrum activity in vitro against gram-positive cocci, except penicillin G-resistant Staphylococcus aureus, and against gram-negative bacilli. It was especially active against Pseudomonas aeruginosa and as active as mezlocillin and piperacillin against Klebsiella pneumoniae. CI-867 was bactericidal against most organisms. Its activity...

Selective medium for Bacillus anthracis.

A need has existed for a selective medium that allows for growth of Bacillus anthracis while inhibiting common contaminants and closely related sporeformers (e.g., B. cereus). A selective medium (PLET medium) was developed containing the following ingredients added to Difco Heart Infusion Agar (HIA): polymyxin, 30 units/ml; lysozyme, 40 ,ug/ml; disodium ethylenediaminetetraacetate (EDTA), 300 ,...

Plague bacillus: survival within host phagocytes.

Pasteurella pestis within nelutrophiles and macrophages removed from the peritoneal cavity of guinea pigs dutring experimental plague were shown to be viable by direct microscopic observation of the infected phagocytes incubating in sutitable bacteriologic media. The time-honored hypothesis that the major determinanit of the virulence of the plague bacillius is its ability to resist ingestion b...